Journal: Scientific Reports

Article Title: Molecular epidemiology and genetic diversity of disappearing Plasmodium vivax in southern Thailand

doi: 10.1038/s41598-025-86578-8

Figure Lengend Snippet: Schematic diagram of ( A ) the Duffy binding protein and ( B ) the merozoite surface protein-1. The black regions indicate the targeted sites of domain II ( pvdbp II ) and the 42-kDa region ( pvmsp1 42 ) selected for amplicon sequencing, with numbers indicating the amino acid boundaries. Shown are the crystal structure of the pvdbp II dimer (PDB ID: 3RRC) and the predicted structure of pvmsp1 42 (AlphaFold ID: F1AQZ6). The target amplicons contain a 395-bp variable region of pvdbp II and a 341-bp variable region of pvmsp1 42 , respectively.

Article Snippet: The COI was determined using the Diversive Amplicon Denoising Algorithms (DADA2), an R package widely used for high-resolution sequence inference in Illumina-based amplicon sequencing data .

Techniques: Binding Assay, Amplification, Sequencing